Tag
His

Part:BBa_I757013:Design

Designed by: Freiburg iGEM 2007 team   Group: iGEM07_Freiburg   (2007-10-24)


His affinity tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions


Source

synthetic DNA by GeneArt

References